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Serum antibodies bound to the coated Fc fragments were detected using a pan anti-human IgG light chain antibody from rabbit (ReMab Biosciences, 32-1031-00) (diluted 1:3000 in PBS/T/S) and visualized by addition of anti-rabbit IgG-HRP (Cytiva Life Sciences, NA935) (diluted 1:3000 in PBS/T/S)