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Ficoll-Paque Plus (Cytiva, Fisher Scientific) was underlaid (10 ml per tube) beneath the cell suspension, followed by centrifugation at 400 g for 40 min at 20 °C (no brake)
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Flavour:Berry 924g
This mechanism triggers the activation of NLRP3 inflammasome by inducing microglial endocytosis and subsequent liberation of lysosomal cathepsin B
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