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Cell culture and stimulation Normal human epidermal keratinocytes (NHEKs) were purchased from Takara Bio (Shiga, Japan) and cultured in Keratinocyte Basal Medium (KBM) overnight prior to human recombinant rIL-22 stimulation (200 ng ml −1 , R&D Systems, Minneapolis, MN)

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FIGURE 2 Investigating ferroptosis not only expands our knowledge of the intricate mechanisms underlying migraines, but it also creates new opportunities for locating possible targets for treatment

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