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coli alkaline phosphatase (AP) that was reduced and inactive, the purified enzyme (200 U, 4 mg) in 50 mM Tris, pH 8.0, was denatured with 3.6 M guanidinium HCl and reduced with 25 mM β-mercaptoethanol under aerobic conditions, as described (Eben and Imlay, 2023)

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