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2.4 Monitoring microbial growth Microbial growth was monitored by direct microscopic counts using a Leica DM2000 LED microscope equipped with a Leica DFC7000 T CCD camera, following staining with SYBR TM Green nucleic acid stain at 1,000× magnification (Invitrogen TM ), following the modified protocol of Summers et al
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110 However, in one large placebo-controlled trial in 2,370 men with infertility, treatment with 30 mg of elemental zinc plus 5 mg (8,500 mcg DFE) folic acid daily for six months increased DNA fragmentation and did not lead to improvements in sperm parameters or pregnancy rate