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Real Time-Polymerase Chain Reaction After drug treatment for 48 h, total RNA of different groups was isolated from middle turn cochlear explants using TRIzol reagent (Life Technologies, 15596026) following manufacturer’s instructions

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I had pain in the injection site but nothing remarkable

The content of GSSG can be determined by first removing GSH from the sample with GSH Scavenger and then applying the aforementioned reaction principle.