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The total length and thickness of GFAP-positive primary astrocytic processes and density of Iba-1–positive microglial cells were assessed using FIJI image analysis software ( as previously reported (Ezquer et al., Experiment 1: Effect of Oral Administration of NAC, ASA, or Their Combination on Chronic Alcohol Intake and mRNA Expression of Nrf2-ARE Regulated Genes in the Hippocampus Twenty-four female UChB rats, weighing 190–210 g were single housed in cages at the age of 60 days and maintained on a 12-h light-dark cycle (lights off at 7:00 PM)
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