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Further, when we examined subcellular localization of the lipid ROS probe by confocal imaging (the probe changes fluorescence from red to green upon oxidation), we found in both HT1080 cells and mouse embryonic fibroblasts (MEFs) treated with erastin, the oxidized probe first appeared in a distribution that significantly colocalized with mitochondria, and then colocalized with the plasma membrane at later time points (Figure 1E and Figure S1)

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