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Accordingly, we examined whether miR-200b expression was affected in OC spheroids

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To evaluate if the endogenous high-molecular weight IL-1β we detected upon inflammasome priming of macrophages represents its ubiquitylation, we stimulated iBMDMs with LPS to induce IL-1β expression, purified the modified IL-1β using GST-UBA, and then treated samples with a ubiquitin specific peptidase, USP21, which cleaves all ubiquitin chain types (Fig