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Protein extraction and western blot analyses Serum-starved NP cells were treated with without 5 μmol·L −1 of the TGFβ R1 kinase inhibitor, LY364947, for 2 h and then stimulated with conditioned media from DFbs for 30 mins

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this systemic imbalance in copper homeostasis constitutes the initiating factor for pulmonary microvascular endothelial cell injury (99)

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