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The bacterial pellet was then resuspended in 800 μL of GMML liquid medium (M9 salts, 1% glycerol, 1 mM MgSO 4 and 0.1 mM CaCl 2 and 0.3 mM Leucine) containing Amp, Spe, IPTG (0.5 mM) and a specific ncAA (1 mM), This suspension was then transferred to 96-deep-well plates (2.2 mL) and incubated at 37 °C, 220 rpm for 11 h, with monitoring of fluorescence intensity (excitation 485 nm and emission 515 nm) as well as OD 600 by Microplate Readers
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