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Pathway enrichment analysis revealed that these genes were enriched in 170 signaling pathways, and the top 20 signaling pathways most closely related to AD were the following (Supplementary Table 2): AD-related pathways include neuroactive ligand-receptor interaction, PI3K-Akt signaling pathway, calcium signaling pathway, cAMP signaling pathway, MAPK signaling pathway, Ras signaling pathway, serotonergic synapse, pathways of neurodegeneration multiple diseases, Rap1 signaling pathway, AD and AGE-RAGE signaling pathway in diabetic complications