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PCLS are now widely utilized to assess integrated cellular responses triggered by inflammatory, fibrotic, or infectious stimuli, as well as for investigating lung damage and regeneration ( in vivo exposed lungs and PCLS behavior and sensitivity to a wide panel of stimuli (physical, mechanical, chemical, pharmacological)

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After fixation, the cells were washed with BD Perm/Wash™ (BD Biosciences), centrifuged, and resuspended in PBS-BSA 0.5% with 0.02% sodium azide for measurements in a FACS Canto II ® , using the FACS Diva software