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GSH + R → GS-R (addition) GSH + R-X → GS-R + HX (displacement of an electron withdrawing group) The major biological function of GST is to provide defense against electrophilic chemical species
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39 For immunohistochemistry, the tissue sections were sequentially placed in xylene I for 15 mins, xylene II for 15 mins, anhydrous ethanol I for 5 mins, anhydrous ethanol II for 5 mins, 85% alcohol for 5 mins, and 75% alcohol for 5 mins, and then were finally washed using distilled water
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