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For the reconstitution of Δ egtA AfS77 , a functional copy of egtA (a 5691 bp PCR-amplified fragment generated with the primers oAetgA1.7_f and oAegtA1.8_r) was subcloned into pAN8.1 containing the phleomycin resistance cassette ( ble ) by a digestion with Sph I and Nhe I resulting in (p)egtA:ble
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doi: 10.1073/pnas.0510352103 40
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