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To prepare the NbLumSyt1-HALO/JF549 complex, a 110-time molar excess of NbLumSyt1-HALO was mixed with JaneliaFluor 549 HaloTag ligand for super-resolution microscopy (Promega, GA1110) at room temperature (i.e., 22-25°C) for 30 min, covered from light, in gentle rotation (20 rpm)
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