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Direct addition of different concentrations of nephrotoxic agents such as ochratoxin A, citrinin, furosemide, and potassium chromate to the suspension releases enzymes specific to the proximal tubule (Table 4), such as alanine aminopeptidase, leucine aminopeptidase and alkaline phosphatase, to the incubation medium in a dosedependent manner (Endou et al., 1985)

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This duration often applies when evaluating acute injury recovery or inflammation reduction