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Xu, Hou, Li, and colleagues, publishing in Bioengineered in 2022, examined the effect of NNMT on lipid accumulation in 3T3-L1 adipocytes using lentiviral RNAi knockdown (NNMT reduced to approximately 27% of control mRNA) and plasmid-driven overexpression, and found that NNMT knockdown significantly reduced lipid accumulation (1.000 ± 0.004 vs