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Kinetic parameters for G3P were determined in 50 mM HEPES-KOH pH 6.5, 100 mM KCl, 0.06 mM DCPIP, with 0.35 µg of Saci_2032 or 0.5 ug of Saci_1118 at 70 °C and varying concentrations of G3P (0–0.3 mM)
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The researchers showed that FOXO4-DRI treatment induced apoptosis specifically in senescent chondrocytes while sparing healthy proliferating cells, confirming the selectivity mechanism described in the original Baar et al
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