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Dissociated hippocampal neurons were transfected with mRFP- ⁇ -actin and shMet RNA and receptor knock-down was allowed to take place for 48 hours prior to stimulating with 0.5 ⁇ g (per well) HGF (10 ng/ml), Dihexa or Nle1-AngIV (both at 10 ⁇ 12 M)

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