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The protein was eluted (elution buffer: 50 mM Tris pH 8, 300 mM NaCl and 10 mM reduced glutathione) and the eluted fractions were pooled and concentrated using Pierce Protein Concentrators PES, 30 K MWCO (88522, Thermo) to 1 ml before size-exclusion chromatography (Cytiva Superdex 75) with SEC buffer (50 mM HEPES pH 7.5, 300 mM KOAc, 5 mM Mg(OAc) 2 , 5% glycerol and 1 mM TCEP)

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RNA interference-mediated silencing of Sod2 in Drosophila leads to early adult-onset mortality and elevated endogenous oxidative stress